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[1]周雨晨,党志雄,蔡佳乐,等.玉米醇溶蛋白基芦丁颗粒的构建、表征及稳定性分析[J].武汉工程大学学报,2025,47(02):187-193.[doi:10.19843/j.cnki.CN42-1779/TQ.202412001]
　ZHOU Yuchen,DANG Zhixiong,CAI Jiale,et al.Preparation, characterization, and stability analysis of zein-based rutin nanoparticles[J].Journal of Wuhan Institute of Technology,2025,47(02):187-193.[doi:10.19843/j.cnki.CN42-1779/TQ.202412001]
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玉米醇溶蛋白基芦丁颗粒的构建、表征及稳定性分析
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
47
期数:
2025年02期
页码:
187-193
栏目:
生物与环境工程
出版日期:
2025-05-09

文章信息/Info

Title:
Preparation, characterization, and stability analysis of zein-based rutin nanoparticles
文章编号:
1674 - 2869（2025）02 - 0187 - 07
作者:
武汉工程大学环境与生态工程学院，绿色化工过程教育部重点实验室（武汉工程大学），国家磷资源开发利用工程技术研究中心，新型反应器与绿色化学工艺湖北省重点实验室，湖北 武汉 430205
Author(s):
School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Key Laboratory of Green
Chemical Process of Ministry of Education (Wuhan Institute of technology), Engineering Research Center of Phosphorus
关键词:
Keywords:
分类号:
TQ460
DOI:
10.19843/j.cnki.CN42-1779/TQ.202412001
文献标志码:
A
摘要:
为了提高芦丁的生物利用率，以玉米醇溶蛋白为载体采用反溶剂法制备包载芦丁的玉米醇溶蛋白纳米颗粒，并对其进行表征。研究结果表明：采用不同的玉米醇溶蛋白和芦丁质量比制备的纳米颗粒粒径都小于200 nm；当玉米醇溶蛋白与芦丁质量比为7∶1时，芦丁的包载率达到最高（50.50%）；Docking分子对接和红外光谱分析表明，玉米醇溶蛋白与芦丁结合的主要驱动力是氢键、静电和疏水作用力。此外，玉米醇溶蛋白芦丁纳米颗粒在远离等电点和高盐时稳定性较好。
Abstract:
To improve the bioavailability of rutin, the antisolvent precipitation method was employed to prepare zein nanoparticles encapsulatin with rutin, and the properties of nanoparticles were characterized and analyzed. The results showed that the average particle sizes of all nanoparticles with different mass ratio of zein to rutin were under 200 nm in diameter. When the mass ratio of zein to rutin was 7∶1, the encapsulation efficiency of rutin reached the highest level (50.50%). Notably, both molecules docking analysis and Fourier transform infrared spectrometry confirmed that rutin and zein might interact through hydrogen bonding, electrostatic and hydrophobic interactions. Moreover, the stability of the nanoparticles was significantly enhanced when they were kept away from the isoelectric point and high-salt environments.

参考文献/References:

[1] 李珂, 冯亚莉, 曹瑞梅, 等. 芦丁的结构修饰及生物活性研究进展[J]. 中草药, 2021,52(20):6413-6424.
[2] 何永艳, 冯佰利, 邓涛, 等. 荞麦提取物抗氧化活性研究[J]. 西北农业学报, 2007(6): 76-79，84.
[3] 褚旭芳, 宋顺喆, 王笑楠, 等. 芦丁与消化系统炎症性疾病关系的研究进展[J]. 医学综述, 2019, 25(20): 4122-4127.
[4] GULLóN B, Lú-CHAU T A, MOREIRA M T, et al. Rutin: a review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability[J]. Trends in Food Science & Technology, 2017, 67: 220-235.
[5] 黄一可. 姜黄素和芦丁微波法提取、纳米载药体系构建及其抗氧化活性的研究[D]. 重庆：重庆医科大学, 2017.
[6] WONG K H,LU A,CHEN X,et al. Natural ingredient- based polymeric nanoparticles for cancer treatment[J]. Molecules, 2020, 25(16): 3620.
[7] GITERU S G, ALI M A, OEY I. Recent progress in understanding fundamental interactions and applications of zein[J]. Food Hydrocolloids, 2021, 120: 106948.
[8] KOLAHI P, SHEKARCHIZADEH H, NASIRPOUR A. Stabilization of pickering emulsion using tragacanth nanoparticles produced by a combination of ultrasonic and anti‐solvent methods[J]. Journal of the Science of Food and Agriculture, 2022, 102(4): 1353-1362.
[9] LI H, XU Y, SUN X, et al. Stability, bioactivity, and bioaccessibility of fucoxanthin in zein-caseinate composite nanoparticles fabricated at neutral pH by antisolvent precipitation[J]. Food Hydrocolloids, 2018, 84: 379-388.
[10] LI J P, LI L. Effect of extrusion temperature on the structure and emulsifying properties of soy protein isolate-oat β-glucan conjugates formed during high moisture extrusion[J]. Food Chemistry, 2023, 429: 136787.
[11] GAGLIARDI A, PAOLINO D, COSTA N, et al. Zein- vs PLGA-based nanoparticles containing rutin: a comparative investigation[J]. Materials Science and Engineering: C, 2021, 118: 111538.
[12] ZHANG X Y,MA D H,YIN C P,et al. The biological activity, functionality, and emulsion stability of soybean meal hydrolysate-proanthocyanidin conjugates[J]. Food Chemistry, 2024, 432: 137159.
[13] MENG R, WU Z Z, XIE Q T, et al. Preparation and characterization of zein/carboxymethyl dextrin nanoparticles to encapsulate curcumin: physicochemical stability, antioxidant activity and controlled release properties[J]. Food Chemistry, 2021, 340: 127893.
[14] HE W Y, TIAN L, ZHANG S S, et al. A novel method to prepare protein-polysaccharide conjugates with high grafting and low browning: application in encapsulating curcumin[J]. Food Science and Technology, 2021, 145: 111349.
[15] LI J P, YANG J J, LI J Z, et al. Structure and rheological properties of extruded whey protein isolate: impact of inulin[J]. International Journal of Biological Macromolecules, 2023, 226: 1570-1578.
[16] POLAT K?SE L, K?SE A. Identification of hCA I, hCA II, AChE and BChE inhibitory properties of some norcantharimide derivatives; molecular docking, SAR and in silico ADME studies[J]. Chemistry Select, 2024, 9(16): e202400684.
[17] ASFOUR M H, MOHSEN A M. Formulation and evaluation of pH-sensitive rutin nanospheres against colon carcinoma using HCT-116 cell line[J]. Journal of Advanced Research, 2018, 9: 17-26.
[18] BHATTACHARJEE S. DLS and zeta potential - what they are and what they are not?[J]. Journal of Controlled Release, 2016, 235: 337-351.
[19] MURDOCK R C, BRAYDICH-STOLLE L, SCHRAND A M, et al. Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique[J]. Toxicological Sciences, 2008, 101(2):239-253.
[20] CHEN H L, XU B G, ZHOU C S, et al. Multi-frequency ultrasound-assisted dialysis modulates the self-assembly of alcohol-free zein-sodium caseinate to encapsulate curcumin and fabricate composite nanoparticles[J]. Food Hydrocolloids, 2022, 122: 107110.
[21] REN X F, ZHANG X, LIANG Q F, et al. Effects of different working modes of ultrasound on structural characteristics of Zein and ACE inhibitory activity of hydrolysates[J]. Journal of Food Quality, 2017：7896037.
[22] SKRT M, BENEDIK E, PODLIPNIK ?, et al. Interactions of different polyphenols with bovine serum albumin using fluorescence quenching and molecular docking[J]. Food Chemistry, 2012, 135(4): 2418-2424.
[23] SHUKLA R, CHERYAN M. Zein: the industrial protein from corn[J]. Industrial Crops and Products, 2001, 13(3): 171-192.
[24] LIU F, MA C, MCCLEMENTS D J, et al. A comparative study of covalent and non-covalent interactions between zein and polyphenols in ethanol-water solution[J]. Food Hydrocolloids, 2017,63: 625-634.
[25] CHUACHAROEN T, SABLIOV C M. Stability and controlled release of lutein loaded in zein nanoparticles with and without lecithin and pluronic F127 surfactants[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2016, 503: 11-18.
[26] LI H, YUAN Y K, ZHU J X, et al. Zein/soluble soybean polysaccharide composite nanoparticles for encapsulation and oral delivery of lutein[J]. Food Hydrocolloids, 2020, 103: 105715.
[27] MWANGI W W, HO K W, TEY B T, et al. Effects of environmental factors on the physical stability of pickering-emulsions stabilized by chitosan particles[J]. Food Hydrocolloids, 2016, 60: 543-550.
[28] ZHAO T T, HUANG L, LUO D H, et al. Fabrication and characterization of anchovy protein hydrolysates-polyphenol conjugates with stabilizing effects on fish oil emulsion[J]. Food Chemistry,2021,351: 129324.
[29] COOPER E A, KNUTSON K. Fourier transform infrared spectroscopy investigations of protein structure[J]. Pharmaceutical Biotechnology, 1995, 7: 101-143.
[30] REMANAN M K, ZHU F. Encapsulation of rutin using quinoa and maize starch nanoparticles[J]. Food Chemistry, 2021, 353: 128534.
[31] BARTH A. Infrared spectroscopy of proteins[J]. Biochimica et Biophysica Acta (BBA) - Bioenerge-tics, 2007, 1767(9): 1073-1101.
[32] MENG R, WU Z, XIE Q T, et al. Preparation and characterization of zein/carboxymethyl dextrin nanoparticles to encapsulate curcumin: physicochemical stability, antioxidant activity and controlled release properties[J]. Food Chemistry, 2021, 340: 127893.
[33] SCHMIDT V， GIACOMELLIC，SOLDI V. Thermal stability of films formed by soy protein isolate-sodium dodecyl sulfate[J]. Polymer Degradation and Stability, 2005, 87(1): 25-31.
[34] SAIKIA S, BORDOLOI M. Molecular docking: challenges, advances and its use in drug discovery perspective[J]. Current Drug Targets, 2019, 20(5): 501-521.

相似文献/References:

备注/Memo

备注/Memo:
收稿日期：2024-12-02
基金项目：国家自然科学基金（31701604）；磷资源开发利用教育部工程研究中心开放/创新基金（GCP202103）；绿色化工过程教育部重点实验室开放项目（GCP202103）
作者简介：周雨晨，硕士研究生。Email：a528051474@163.com
*通信作者：杨 芳，博士，教授。Email：yangfang2001@126.com
引文格式：周雨晨，党志雄，蔡佳乐，等 . 玉米醇溶蛋白基芦丁颗粒的构建、表征及稳定性分析［J］. 武汉工程大学学报，2025，47（2）：187-193.
更新日期/Last Update: 2025-05-08