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[1]丁寅莹,严文煜,罗晓刚*. 水样中邻苯二酚和间苯二酚的辣根过氧化物酶比色试纸测定 [J].武汉工程大学学报,2025,47(04):355-362.[doi:10.19843/j.cnki.CN42-1779/TQ.202303032]
　DING Yinying,YAN Wenyu,LUO Xiaogang*. Horseradish peroxidase-immobilized colorimetric test strips for detection of catechol and resorcinol in water samples [J].Journal of Wuhan Institute of Technology,2025,47(04):355-362.[doi:10.19843/j.cnki.CN42-1779/TQ.202303032]
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水样中邻苯二酚和间苯二酚的辣根过氧化物酶比色试纸测定
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
47
期数:
2025年04期
页码:
355-362
栏目:
现代大化工
出版日期:
2025-08-29

文章信息/Info

Title:
Horseradish peroxidase-immobilized colorimetric test strips for detection of catechol and resorcinol in water samples
文章编号:
1674 - 2869（2025）04 - 0355- 08
作者:
1.武汉工程大学化工与制药学院，绿色化工过程教育部重点实验室（武汉工程大学），新型反应器与绿色化学工艺湖北省重点实验室（武汉工程大学），湖北 武汉 430205；
2.湖北三峡实验室，湖北 宜昌 443008
Author(s):
1. Key Laboratory for Green Chemical Process of Ministry of Education（Wuhan Institute of Technology）, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology（Wuhan Institute of Technology）, School of Chemical Engineering and
Pharmacy, Wuhan Institute of Technology, Wuhan 430205, China; 2. Hubei Three Gorges Laboratory, Yichang 443008, China
关键词:
Keywords:
分类号:
O636.1+1
DOI:
10.19843/j.cnki.CN42-1779/TQ.202303032
文献标志码:
A
摘要:
开发现场高效的检测水体中邻苯二酚和间苯二酚的平台具有重要意义。以纤维素的溶解再生成膜为基础，化学修饰后引入活性生物酶，制备成具有生物活性的纤维素膜基比色试纸，以探究其在水生环境中的有毒有害物质的及时检测。以纤维素膜为载体，高碘酸钠氧化法成功将纤维素结构C2、C3位的羟基氧化为醛基，得到氧化纤维素膜。通过席夫碱反应将辣根过氧化物酶成功固定在羧基化纤维素膜表面。通过扫描电镜展现了纤维素纯膜、氧化纤维素膜、载酶纤维素膜的表面形貌与特征。能谱扫描仪、红外光谱等表征分析了三种膜的表面元素构成和官能团类型。设计了一系列应用实验，包括实验条件的优化、标准溶液检测、检测时间的探究等，系统研究了纤维素膜基比色试纸对环境水样中邻苯二酚和间苯二酚的检测性能。实验证明本项工作制备的纤维素膜基比色试纸对邻苯二酚和间苯二酚的最低检测限分别为0.45、0.09?mmol/L，具有较好的稳定性、便利的及时检测性，在生物传感和环境监测领域具有广阔的应用前景。
Abstract:
Developing an efficient on-site detection platform for catechol and resorcinol in water is crucial for environmental monitoring. Using dissolution and regeneration of cellulose membranes, we introduced active biological enzymes after chemical modification to prepare enzyme-immobilized cellulose membrane-based colorimetric test strips for rapid detection of toxic substances in aquatic environments. The cellulose membrane was functionalized by oxidizing the hydroxyl groups at the C2 and C3 positions to aldehyde groups using sodium periodate oxidation, yielding oxidized cellulose membranes. Horseradish peroxidase (HRP) was then immobilized on the carboxylated cellulose membrane surface via Schiff base reaction. Scanning electron microscopy (SEM) revealed the surface morphology of native cellulose, oxidized cellulose, and HRP-immobilized membranes. Energy-dispersive X-ray spectroscopy (EDS) and Fourier-transform infrared spectroscopy (FT-IR) characterized the elemental composition and functional groups of these membranes. We conducted systematic experiments including optimization of detection conditions, calibration with standard solutions, and response time evaluation. The test strips demonstrated detection limits of 0.45 mmol/L for catechol and 0.09 mmol/L for resorcinol, with excellent stability and rapid response, showing great potential for biosensing and environmental monitoring applications.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2023-03-25
基金项目：国家自然科学基金(51773159)
作者简介：丁寅莹，硕士研究生。Email：876679589@qq.com
*通信作者：罗晓刚，博士，教授。Email：xgluo0310@hotmail.com
引文格式：丁寅莹，严文煜，罗晓刚. 水样中邻苯二酚和间苯二酚的辣根过氧化物酶比色试纸测定［J］. 武汉工程大学学报，2025，47（4）：355-362.
更新日期/Last Update: 2025-08-29