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[1]余心洁,樊昊心*.稳定性同位素探针技术及其微生物生态学应用[J].武汉工程大学学报,2025,47(05):531-538.[doi:10.19843/j.cnki.CN42-1779/TQ.202404001]
　YU Xinjie,FAN Haoxin*.DNA-based stable isotope probing and its application in microbial ecology[J].Journal of Wuhan Institute of Technology,2025,47(05):531-538.[doi:10.19843/j.cnki.CN42-1779/TQ.202404001]
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稳定性同位素探针技术及其微生物生态学应用
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
47
期数:
2025年05期
页码:
531-538
栏目:
现代大化工
出版日期:
2025-10-31

文章信息/Info

Title:
DNA-based stable isotope probing and its application in microbial ecology
文章编号:
1674 - 2869（2025）05 - 0531 - 08
作者:
武汉工程大学环境生态与生物工程学院，湖北 武汉 430205
Author(s):
School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China
关键词:
Keywords:
分类号:
S154.3
DOI:
10.19843/j.cnki.CN42-1779/TQ.202404001
文献标志码:
A
摘要:
稳定性同位素核酸探针技术（DNA-SIP）是近20年来发展起来的一种新兴技术，结合了同位素示踪和分子生物学分类的优势，能够在环境中分离鉴定代谢活跃的功能微生物。首先介绍了DNA-SIP的基本原理，包括稳定同位素底物的选择、培养条件设定及下游分子分析方法，并讨论了高通量测序与生物信息学发展对分辨率和灵敏度提升的重要作用。在此基础上，综述了DNA-SIP在微生物生态学中的应用进展：一是在碳、氮、硫等元素生物地球化学循环中揭示了关键功能类群；二是在根际微生物生态研究中阐明了植物-微生物互作及其对养分吸收的贡献；三是在污染微生物生态学中鉴定了多种有机污染物的主要降解者。尽管DNA-SIP面临交叉喂养、同位素灵敏度差异和数据分析复杂等挑战，结合宏基因组学及单细胞拉曼光谱等新技术将进一步扩展其应用前景。DNA-SIP为解析复杂环境中微生物的功能作用提供了独特而强有力的工具，在环境科学与微生物生态学研究中展现出广阔的发展潜力。
Abstract:
DNA-based stable isotope probing (DNA-SIP) is an emerging technique developed over the past two decades that combines the strengths of isotope tracing and molecular biology, enabling the isolation and identification of metabolically active microorganisms in complex environments. This review first outlines the fundamental principles of DNA-SIP, including the selection of isotope-labeled substrates, cultivation conditions, and downstream molecular analysis methods, and highlights the importance of advances in high-throughput sequencing and bioinformatics for improving resolution and sensitivity. Building on this foundation, we summarized recent applications of DNA-SIP in microbial ecology: (i) revealing key functional groups involved in biogeochemical cycles of carbon, nitrogen, and sulfur; (ii) elucidating plant-microbe interactions in the rhizosphere and their contribution to nutrient acquisition; and (iii) identifying major degraders of organic pollutants in contaminated environments. Despite challenges such as cross-feeding, differential isotope incorporation, and complex data analysis, the integration of DNA-SIP with metagenomics, single-cell Raman spectroscopy, and other emerging approaches is expected to further expand its utility. Overall, DNA-SIP provides a powerful and unique tool for disentangling microbial functions in situ and holds broad prospects for advancing research in environmental science and microbial ecology.

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备注/Memo

备注/Memo:
收稿日期：2024-04-01
基金项目：国家自然科学基金（4207039）
作者简介：余心洁，硕士研究生。Email：570371638@qq.com
*通信作者：樊昊心，博士，副教授。Email：haoxin.fan@wit.edu.cn
引文格式：余心洁，樊昊心. 稳定性同位素探针技术及其微生物生态学应用［J］. 武汉工程大学学报，2025，47（5）：531-538.
稳定性同位素探针技术及其微生物生态学应用


更新日期/Last Update: 2025-11-03