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[1]金国庆,李 瑞,樊昊心,等.PBAT海洋降解菌的筛选及其降解特性[J].武汉工程大学学报,2025,47(05):524-530.[doi:10.19843/j.cnki.CN42-1779/TQ. 202404003]
　JIN Guoqing,LI Rui,FAN Haoxin,et al.Isolation and characterization of marine bacteria capable of degrading PBAT[J].Journal of Wuhan Institute of Technology,2025,47(05):524-530.[doi:10.19843/j.cnki.CN42-1779/TQ. 202404003]
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PBAT海洋降解菌的筛选及其降解特性
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
47
期数:
2025年05期
页码:
524-530
栏目:
现代大化工
出版日期:
2025-10-31

文章信息/Info

Title:
Isolation and characterization of marine bacteria capable of degrading PBAT
文章编号:
1674 - 2869（2025）05 - 0524 - 07
作者:
武汉工程大学环境生态与生物工程学院，湖北 武汉 430205
Author(s):
School of Environmental Ecology and Biological engineering，Wuhan Institute of Technology，Wuhan 430205，China
关键词:
Keywords:
分类号:
S182
DOI:
10.19843/j.cnki.CN42-1779/TQ. 202404003
文献标志码:
A
摘要:
为筛选和鉴定海洋环境中能够有效降解聚己二酸/对苯二甲酸丁二酯（PBAT）的微生物资源，研究了在中国广东省周边海域，海洋环境中富集的PBAT生物膜，通过分离筛选方法，成功获得3株具有潜在降解能力的菌种，分别为芽孢杆菌属（Bacillus sp.）、嗜冷杆菌属（Psychrobacter sp.）和巨大普利斯特菌属（Priestia sp.）。在含有2216 E作为额外碳源的降解实验中，经过45 d的培养，3种菌株均展现出对PBAT的降解能力，其中芽孢杆菌属菌株的降解效率最高，降解率为（0.97±0.09）%，而嗜冷杆菌属和巨大普利斯特菌属的降解率相近，分别为（0.590±0.190）%和（0.590±0.002）%。通过扫描电镜（SEM）观察到3种菌株均能在PBAT表面形成显著的粗糙结构，产生多处沟壑，表明微生物对PBAT表面有侵蚀作用。此外，傅里叶红外光谱（FTIR）分析发现PBAT结构中的特征峰发生了伸缩振动，表明PBAT的化学结构在微生物作用下发生了变化。这些结果为进一步研究和开发利用微生物降解PBAT提供了重要的基础数据。
Abstract:
This study aimed to screen and identify microbial resources in the marine environment that are capable of efficiently degrading poly (butylene adipate-co-terephthalate) (PBAT). ?PBAT biofilms were collected from the seas surrounding Guangdong Province, China. Three bacterial strains with potential degrading capabilities were isolated: Bacillus sp., Psychrobacter sp., and Priestia sp. In degradation experiments using 2216 E medium as an additional carbon source, all three strains degraded PBAT after 45 days of incubation. The Bacillus sp. strain?exhibited the highest degradation efficiency (0.97 ± 0.0)9% , whereas Psychrobacter sp. and Priestia sp. showed similar, lower rates of （0.590±0.190)% and （0.590 ± 0.002)%, respectively. Scanning electron microscopy (SEM) revealed that all three strains formed biofilms and created grooves and erosions on the PBAT surface. Furthermore, Fourier-transform infrared spectroscopy (FTIR) detected changes in the characteristic peaks of PBAT, indicating alterations in its chemical structure due to microbial activity. These results provide fundamental data for further research and development on microbial degradation of PBAT.

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备注/Memo

备注/Memo:
收稿日期：2024-04-01
基金项目：国家自然科学基金（4207039）
作者简介：金国庆，硕士研究生。Email：jin990426@163.com
*通信作者：葛超荣，博士，教授。Email：chaorongge@163.com
引文格式：金国庆，李瑞，樊昊心，等. PBAT海洋降解菌的筛选及其降解特性［J］. 武汉工程大学学报，2025，47（5）：524-530.

更新日期/Last Update: 2025-11-03