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[1]赵 锦,吴有斌.微生物来源青霉素酰化酶固定化载体构建研究进展 [J].武汉工程大学学报,2026,48(04):418-425.[doi:10.19843/j.cnki.CN42-1779/TQ.202605014]
　ZHAO Jin,WU Youbin. Research progress on the construction of immobilization carriers for microbe-derived penicillin G acylase [J].Journal of Wuhan Institute of Technology,2026,48(04):418-425.[doi:10.19843/j.cnki.CN42-1779/TQ.202605014]
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微生物来源青霉素酰化酶固定化载体构建研究进展
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
48
期数:
2026年04期
页码:
418-425
栏目:
现代大化工
出版日期:
2026-08-30

文章信息/Info

Title:
Research progress on the construction of immobilization carriers for microbe-derived penicillin G acylase
文章编号:
1674 - 2869（2026）04 - 0418 - 08
作者:
赵 锦1; 吴有斌2
1. 湖北轻工职业技术学院轻化工程学院，湖北 武汉 430070；
2. 宜昌人福药业有限责任公司产品开发中心，湖北 宜昌 443000
Author(s):
ZHAO Jin1; WU Youbin2
1. School of Light Chemical Engineering, Hubei Light Industry Technology Institute, Wuhan 430070， China；
2. R&D Department， Yichang Humanwell Pharmaceutical Co., Ltd.， Yichang 443000， China
关键词:
青霉素酰化酶; 固定化载体; 表面修饰; 金属有机骨架; 酶学性能; 工业生物催化
Keywords:
penicillin G acylase (PGA);  immobilization carrier;  surface modification;  metal-organic framework;  enzymatic performance;  industrial biocatalysis
分类号:
TQ465；Q55
DOI:
10.19843/j.cnki.CN42-1779/TQ.202605014
文献标志码:
A
摘要:
青霉素酰化酶（PGA）是6-氨基青霉烷酸及半合成β-内酰胺类抗生素绿色制造中的关键生物催化剂。围绕“结构特征—载体构建—固定化策略—性能评价—工业适配”的逻辑，梳理近年有机高分子、无机/磁性纳米材料、金属有机骨架（MOFs）及天然传统载体研究文献,系统综述微生物源青霉素酰化酶固定化载体的构建相关研究进展。研究表明：亲水高分子载体有利于维持酶分子表面水化层；磁性纳米载体便于快速催化剂回收；MOFs在孔道限域和微环境调控方面具有潜力；连续流和固定床评价正在成为连接材料设计与工业应用的重要方向。当前研究仍存在载体成本偏高、传质限制、酶分子取向调控和长期运行稳定性不足等问题。后续研究需聚焦低成本载体、酶定向固定化、层级孔结构和连续化反应器的协同设计，推动固定化PGA催化剂在β-内酰胺类抗生素绿色制造中的规模化应用。
Abstract:
Penicillin G acylase (PGA) acts as a key biocatalyst for the green manufacturing of 6-aminopenicillanic acid and semi-synthetic β-lactam antibiotics. In this review, we systematically summarized recent progress on PGA immobilization carriers by following a unified framework of “structural characteristics-support fabrication-immobilization strategy-performance evaluation-industrial adaptability”, covering organic polymers, inorganic/magnetic nanomaterials, metal-organic frameworks (MOFs), and traditional natural carriers. Existing studies reveal that hydrophilic polymer carriers help preserve the hydration shell of enzymes; magnetic nanocarriers facilitate rapid catalyst recovery; MOFs exhibit great potential in pore confinement and microenvironment regulation; evaluation via continuous-flow and fixed-bed systems has become a vital bridge between material design and industrial application. Several bottlenecks remain unresolved, including high carrier cost, mass transfer limitation, poor controllability of enzyme orientation, and insufficient stability during long-term operation. Future research should strengthen the collaborative design of low-cost carriers, oriented immobilization, hierarchical pore structures and continuous reactors, so as to promote large-scale application of immobilized PGA in the green production of β-lactam antibiotics.

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备注/Memo

备注/Memo:
收稿日期：2026-05-26
基金项目：全国生物技术职业教育教学指导委员会生物技术领域职业教育教学关键要素改革培育项目（SWXMGJYS 2026045）；湖北省新时代职业学校名师（名匠）名校长培养计划（鄂教职成办函[2026]2号）
作者简介：赵 锦，硕士，副教授。Email：254699465@qq.com
更新日期/Last Update: 2026-09-05