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[1]翟马跃,梁淑彩*,潘 宇,等.亚硝酸根的2,3-二氨基吩嗪荧光猝灭法测定[J].武汉工程大学学报,2016,38(05):431-436.[doi:10. 3969/j. issn. 1674?2869. 2016. 05. 004]
 ZHAI Mayue,LIANG Shucai*,PAN Yu,et al.Determination of Nitrite with 2, 3-Diamino-Phenazine by Fluorescence Quenching Method[J].Journal of Wuhan Institute of Technology,2016,38(05):431-436.[doi:10. 3969/j. issn. 1674?2869. 2016. 05. 004]
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亚硝酸根的2,3-二氨基吩嗪荧光猝灭法测定(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
38
期数:
2016年05期
页码:
431-436
栏目:
材料科学与工程
出版日期:
2016-11-02

文章信息/Info

Title:
Determination of Nitrite with 2, 3-Diamino-Phenazine by Fluorescence Quenching Method
作者:
翟马跃1梁淑彩2*潘 宇1刘羽萍1谭支林1刘 凡1鄢国平1*
1. 武汉工程大学材料科学与工程学院,湖北 武汉 430074;2. 武汉大学药学院,湖北 武汉 430074
Author(s):
ZHAI Mayue1 LIANG Shucai2* PAN Yu1 LIU Yuping1 TAN Zhilin1 LIU Fan1YAN Guoping1*
1. School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430074, China;2. School of Pharmaceutical Sciences, Wuhan University, Wuhan 430074, China
关键词:
荧光分析法亚硝酸盐23-二氨基吩嗪胶束增敏
Keywords:
fluorescence analysisnitrite23-diamino-phenazinemicellar sensitization
分类号:
O657.3
DOI:
10. 3969/j. issn. 1674?2869. 2016. 05. 004
文献标志码:
A
摘要:
2,3-二氨基吩嗪(DAP)在中性环境中具有强荧光. 发现其在酸性介质中能与亚硝酸根离子反应,生成产物在中性环境下荧光很弱,据此建立了一种以DAP为探针,荧光猝灭测定水样中亚硝酸根离子的方法. 在最佳测定条件下(0.03 mol/L 的硫酸,2.0×10-3 mol/L十六烷基三甲基溴化铵,反应温度50 ℃、反应时间50 min,激发和发射波长分别为435 nm和557 nm),体系荧光强度变化与亚硝酸根浓度在0.1 mol/L~2.1×10-6 mol/L 范围内呈线性关系,检测限为9.8×10-8 mol/L. 该方法具有操作简便、灵敏度高、长波长下检测及斯托克位移大的优点.
Abstract:
The 2,3-Diamino-phenazine(DAP) has strong fluorescence in the neutral medium. We found that it could react with nitrite in acidic medium, and the produce displayed weak fluorescence in the neutral medium, thus a fluorimetric quenching method for the detection of nitrite in water samples was developed employing DAP as fluorescence probe. Upon the optimum experimental conditions (0.03 mol/L of H2SO4, 2.0×10-3 mol/L of hexadecyl trimethyl ammonium bromide, reaction temperature of 50 ℃, reaction time of 50 min, fluorescence detected at 557nm with the excitation wavelength of 435 nm), the fluorescence change was linear to nitrite concentration over the range of 0.1 mol/L - 2.1×10-6 mol/L with the detection limit of 9.8×10-8 mol/L. The proposed method possessed the characteristics of simpleness, sensitivity, long detection wavelength and large Stocks shift.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:翟马跃,硕士研究生. E-mail: zmyysyy@163.com*通讯作者:鄢国平,博士,教授. E-mail: guopyan2006@163.com 梁淑彩,博士,副教授. E-mail: chirolab@gmail.com
更新日期/Last Update: 2016-10-31