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[1]黄天悦,高 鹏,王 进,等.γ-聚谷氨酸的发酵优化及其对辣椒生长的影响[J].武汉工程大学学报,2020,42(02):143-152.[doi:10.19843/j.cnki.CN42-1779/TQ. 201904028]
 HUANG Tianyue,GAO Peng,WANG Jin,et al.Fermentation Optimization of Gamma-Polyglutamic Acid and Its Effect on Grow of Pepper[J].Journal of Wuhan Institute of Technology,2020,42(02):143-152.[doi:10.19843/j.cnki.CN42-1779/TQ. 201904028]
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γ-聚谷氨酸的发酵优化及其对辣椒生长的影响(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
42
期数:
2020年02期
页码:
143-152
栏目:
化学与化学工程
出版日期:
2021-01-26

文章信息/Info

Title:
Fermentation Optimization of Gamma-Polyglutamic Acid and Its Effect on Grow of Pepper
文章编号:
1674 - 2869(2020)02 - 0143 - 10
作者:
黄天悦高 鹏王 进张旭松陈 斐陈孝平*
武汉工程大学环境生态与生物工程学院,湖北 武汉 430205
Author(s):
HUANG Tianyue GAO Peng WANG Jin ZHANG Xusong CHEN Fei CHEN Xiaoping*
School of Environmental Ecology and Bioengineering, Wuhan Institute of Technology, Wuhan 430205, China
关键词:
γ-聚谷氨酸发酵优化叶面肥增效剂辣椒产量
Keywords:
γ-polyglutamic acid (γ-PGA) fermentation optimization synergist of leave fertilizer pepper yield
分类号:
Q939.97
DOI:
10.19843/j.cnki.CN42-1779/TQ. 201904028
文献标志码:
A
摘要:
采用实验室分离的一株高产 γ-聚谷氨酸(γ-PGA)枯草芽孢杆菌发酵生产 γ-聚谷氨酸。利用单因素优化实验选择最佳碳源、氮源及发酵前体,响应面分析法Box-Behnken。实验结果表明最佳发酵培养基配方为蔗糖60.39 g /L、硫酸铵6.00 g /L、谷氨酸钠93.27 g /L,γ-PGA产量达到70.285 g/L。将发酵提纯后获得的产品与尿素混合制备成叶面肥对辣椒进行喷施,辣椒的株高、茎粗、挂果数、果重以及叶绿素含量等显著增加,喷施10 g/L 尿素 + 0.6 g/L γ-PGA增产效果最佳,γ-PGA复合肥料的生物可降解性、环境友好性和保肥增效特性有利于农业的可持续发展。
Abstract:
The Bacillus subtilis highly yielding γ-polyglutamic acid (γ-PGA) separated in the laboratory was fermented to produce γ-polyglutamic acid. The single factor optimal experiment was used to optimize carbon source, nitrogen source and precursor of fermentation. The result of response surface methodology experiment show the optimal formula of fermentation medium is sucrose of 60.39 g/L, ammonium sulfate of 6.00 g/L and sodium glutamate of 93.27 g/L, with which the output of γ-PGA increases from 7.253 g/L to 70.285 g/L. The product obtained by fermentation and purification was mixed with carbamide to prepare foliar fertilizer, which was then sprayed to the pepper plant. It was found that the plant height, stem diameter, number of fruits, fruit weight and chlorophyll content of pepper increase significantly. Spraying carbamide of 10 g/L + γ-PGA of 0.6 g/L can produce the best yield-increasing effect. Moreover, the γ-PGA compound fertilizer is beneficial to the sustainable development of agriculture with biodegradability, environmental friendliness and nutrient- preserving capability.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2019-06-08作者简介:黄天悦,硕士研究生。E-mail:1823848552@qq.com*通讯作者:陈孝平,博士,副教授。E-mail:xiao.ping.chen@foxmail.com引文格式:黄天悦,高鹏,王进,等. γ-聚谷氨酸的发酵优化及其对辣椒生长的影响[J]. 武汉工程大学学报,2020,42(2):143-152.
更新日期/Last Update: 2020-06-19